Circ Cardiovasc Imaging. 2014 Apr 28. doi: 10.1161/CIRCIMAGING.113.001806. [Epub ahead of print]

In Vivo Molecular Imaging of Thrombosis and Thrombolysis Using a Fibrin-binding Positron Emission Tomography Probe

Ay I, Blasi F, Rietz TA, Rotile NJ, Kura S, Brownell AL, Day H, Oliveira BL, Looby RJ, Caravan P.


BACKGROUND: -Fibrin is a major component of arterial and venous thrombi and represents an ideal candidate for molecular imaging of thrombosis. Here, we describe imaging properties and target uptake of a new fibrin-specific PET probe for thrombus detection and therapy monitoring in two rat thrombosis models.
METHODS AND RESULTS: -The fibrin-binding probe FBP7 was synthesized by conjugation of a known short cyclic peptide to a cross-bridged chelator (CB-TE2A), followed by labeling with copper-64. Adult male Wistar rats (n=26) underwent either carotid crush injury (mural thrombosis model) or embolic stroke (occlusive thrombosis model) followed by rtPA treatment (10 mg/kg, i.v.). FBP7 detected thrombus location in both animal models with a high PET target-to- BACKGROUND: -We demonstrated that FBP7 is suitable for molecular imaging of thrombosis and thrombolysis in vivo, and represents a very promising candidate for bench-to-bedside translation.

PMID: 24777937