Angew Chem Int Ed Engl. 2015 Oct 26;54(44):13002-6 doi: 10.1002/anie.201505525. 2015 Sep 14.

Heat-Induced Radiolabeling of Nanoparticles for Monocyte Tracking by PET

Normandin MD, Yuan H, Wilks MQ, Chen HH, Kinsella JM, Cho H, Guehl NJ, Absi-Halabi N, Hosseini SM, El Fakhri G, Sosnovik DE, Josephson L.

Abstract

Heat-induced radiolabeling (HIR) yielded (89) Zr-Feraheme (FH) nanoparticles (NPs) that were used to determine NP pharmacokinetics (PK) by positron emission tomography (PET). Standard uptake values indicated a fast hepatic uptake that corresponded to blood clearance, and a second, slow uptake process by lymph nodes and spleen. By cytometry, NPs were internalized by circulating monocytes and monocytes in vitro. Using an IV injection of HIR (89) Zr-FH (rather than in vitro cell labeling), PET/PK provided a view of monocyte trafficking, a key component of the immune response.

PMID: 26368132