Abstract

Magnetic resonance imaging (MRI) is capable of generating high-resolution brain images with fine anatomic detail and unique tissue contrasts that reveal structures that are not visible to the eye. Sharply defined gray- and white-matter interfaces allow for quantitative anatomic analysis that can be accurately performed with largely automated segmentation methods. In an analogous fashion, diffusion MRI in the brain provides structural information based on contrasts derived from the diffusivity of water in brain tissue, which can highlight the orientation of neuronal axons. Also using largely automated methods, diffusion MRI can be used to generate models of white-matter tracts throughout the brain, a method known as tractography, as well as characterize the microstructural integrity of neuronal axons. Tractographic analysis has helped to define connectivity in the brain that powerfully informs understanding of brain function, and, together with other diffusion metrics, is useful in evaluation of the normal and diseased brain. The quantitative methods of brain segmentation, tractography, and diffusion MRI extend MRI into a realm beyond visual inspection and provide otherwise unachievable sensitivity and specificity in the analysis of brain structure and function.